1 1

Croyezbio

C13004-K01

T7 RNA Polymerase ELISA Kit for IVT mRNA QC

T7 RNA Polymerase ELISA Kit for IVT mRNA QC

$1,584.00 USD
$1,584.00 USD
Sale Sold out
Shipping calculated at checkout.
Size
03 — PROCESS RESIDUAL

Quantify Residual T7 RNA Polymerase After IVT

A sensitive sandwich ELISA for quantitative detection of T7 RNA Polymerase in research-use IVT RNA workflows.

LOD
0.037 ng/mL
LOQ
0.124 ng/mL
DETECTION RANGE
0.15625–10 ng/mL
FORMAT
96 Wells

Why Measure Residual T7 RNA Polymerase?

T7 RNA polymerase is commonly used to generate RNA during in vitro transcription.

After transcription and purification, researchers may want to evaluate how much process enzyme remains in the sample as part of a broader process-development or RNA QC workflow.

RNA size, dsRNA impurity and residual T7 polymerase answer three different QC questions.

Sandwich ELISA for T7 RNA Polymerase

The assay uses a monoclonal antibody to capture T7 RNA Polymerase, followed by an HRP-conjugated detection antibody and colorimetric measurement at 450 nm.

1. Sample Containing T7 RNA Polymerase
↓
2. Capture Antibody Binding
↓
3. HRP-Conjugated Detection Antibody
↓
4. OD450 Measurement & Quantification

Applications

IVT Process Development
Evaluate residual T7 polymerase following transcription.
Purification Evaluation
Compare T7 polymerase levels before and after purification.
mRNA Research
Add residual enzyme analysis to an IVT mRNA QC workflow.
saRNA Research
Complement size and impurity analysis for long RNA workflows.

Assay Performance

LIMIT OF DETECTION
0.037 ng/mL
LIMIT OF QUANTIFICATION
0.124 ng/mL
DETECTION RANGE
0.15625–10 ng/mL

Product Specifications

Catalog Number C13004-K01
Product T7 RNA Polymerase ELISA Kit
Format Pre-coated 96-well microplate (12 × 8-well strips)
Assay Sandwich ELISA
Target T7 RNA Polymerase
LOD 0.037 ng/mL
LOQ 0.124 ng/mL
Detection Range 0.15625–10 ng/mL
Readout 450 nm
Storage 2–8°C

Complete Your IVT RNA QC Workflow

01 — SIZE & INTEGRITY

12K RNA Ladder

Evaluate RNA size with high-range reference bands.

View CR00005 →
02 — dsRNA IMPURITY

dsRNA ELISA

Quantitatively evaluate residual double-stranded RNA.

View C13005 →
03 — PROCESS RESIDUAL

T7 RNA Polymerase ELISA

Quantitative residual enzyme analysis following IVT.

You are here
RNA QC RESOURCES

Build a Practical IVT RNA QC Workflow

See how RNA size, dsRNA impurity and residual T7 RNA polymerase can be evaluated as complementary QC questions.

→ Read the IVT mRNA QC Workflow Guide
DANA BIOSCIENCE RNA QC SOLUTIONS

Add Residual Enzyme Analysis to Your IVT Workflow

Combine T7 RNA polymerase analysis with RNA size and dsRNA impurity assessment for a broader view of your IVT RNA process.

Explore RNA QC Solutions

For Research Use Only. Not for diagnostic or therapeutic use. Follow the manufacturer's product manual for assay conditions, sample preparation and interpretation.

View full details