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Croyezbio

CR00005-50UL

12K High Range RNA Ladder for Long RNA, mRNA & saRNA – 200 nt to 12 kb

12K High Range RNA Ladder for Long RNA, mRNA & saRNA – 200 nt to 12 kb

$320.00 USD
$320.00 USD
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Size
LONG RNA • mRNA • saRNA

See Beyond Conventional RNA Ladder Ranges

A high-range ssRNA size standard covering 200 nt to 12,000 nt for electrophoretic analysis of long RNA, IVT mRNA and self-amplifying RNA research samples.

RANGE
0.2–12 kb
FORMAT
9 ssRNA Bands
SIZE
50 µL
STORAGE
−80°C

Nine Reference Bands Up to 12 kb

The 12K High Range RNA Ladder contains nine single-stranded RNA fragments generated by in vitro transcription from linear DNA templates.

RNA REFERENCE SIZES
0.2 · 0.5 · 1 · 2 · 3 · 4 · 6 · 9 · 12 kb

Why Use a 12K RNA Ladder?

Many conventional RNA markers are designed for shorter transcripts. When working with long IVT RNA or saRNA constructs, the expected transcript may extend beyond those reference ranges.

Shorter-Range Ladder

An 8–10 kb RNA sample may migrate above the highest available reference band.

12K High Range Ladder

9 kb and 12 kb reference bands provide useful comparison points for larger RNA constructs.

Applications

Long IVT RNA
Size assessment of larger in vitro transcribed RNA constructs.
mRNA Research
Electrophoretic comparison of mRNA transcript size.
saRNA Research
High-range reference bands for longer self-amplifying RNA constructs.
RNA Electrophoresis
ssRNA size reference on native agarose gels.

Product Specifications

Catalog Number CR00005-50UL
Product 12K High Range RNA Ladder
Package 50 µL
RNA Type Single-stranded RNA
Range 200 nt–12,000 nt
Application Native agarose gel electrophoresis
Storage Buffer 1 mM sodium citrate, pH 6.4
Storage −80°C; avoid repeated freeze/thaw cycles

RNA Ladder Preparation

1. Mix 1 volume of RNA ladder with 3 volumes of 2× RNA Loading Dye.
2. Incubate at 65°C for 5 minutes.
3. Immediately cool on ice before loading according to the recommended protocol.

Build a More Complete IVT RNA QC Workflow

RNA size is only one part of IVT RNA quality control.

STEP 1

RNA Size & Integrity

12K RNA Ladder

Compare your RNA with known size references.

STEP 2

dsRNA Impurity

J2-based dsRNA ELISA

Quantitatively compare residual dsRNA in IVT samples.

STEP 3

Residual Enzyme

T7 RNA Polymerase ELISA

Evaluate residual T7 RNA polymerase after purification.

RNA QC RESOURCES

Need help designing your RNA QC workflow?

Learn how to select an RNA ladder, understand dsRNA impurities, and build a practical IVT RNA QC workflow.

→ RNA Ladder Selection Guide: 6K vs 9K vs 12K vs 15K

→ Why dsRNA Impurities Matter in IVT mRNA & saRNA

→ A Practical IVT mRNA QC Workflow

DANA BIOSCIENCE RNA QC SOLUTIONS

Working With Long RNA or saRNA?

Explore complementary tools for RNA size, dsRNA impurity and IVT process-related QC.

Explore RNA QC Solutions

For Research Use Only. The High Range RNA Ladder is intended as an RNA size standard and is not recommended for use as a quantitative RNA standard.

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