YTHDC1 Antibody

Size

100ug

Clone Number

Aliases

YTHDC1 antibody; KIAA1966 antibody; YT521 antibody; YTH domain-containing protein 1 antibody; Splicing factor YT521 antibody; YT521-B antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q96MU7

Immunogen

Recombinant Human YTH domain-containing protein 1 protein (89-153AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB; Recommended dilution: WB:1:1000-1:5000

Background

Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed:26318451, PubMed:26876937, PubMed:25242552). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:26318451, PubMed:25242552). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937). May also regulate alternative splice site selection (PubMed:20167602).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

YTHDC1

Research Areas

Epigenetics and Nuclear Signaling