• EKLF Polyclonal Antibody
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Upingbio

YP-Ab-02245-100UL

EKLF Polyclonal Antibody

EKLF Polyclonal Antibody

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  • Gene Name: KLF1
  • Immunogen: The antiserum was produced against synthesized peptide derived from human KLF1 around the non-acetylation site of Lys274. AA range:231-280
  • Specificity: EKLF Polyclonal Antibody detects endogenous levels of EKLF protein only when acetylation at K274.
  • Composition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Source: Polyclonal, Rabbit,IgG
  • Dilution ratio: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
  • Purification process: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration: 1 mg/ml
  • Storage: -20°C/1 year
  • Other Names: KLF1; EKLF; Krueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF
  • Background: This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009],
  • Function: function:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation, acts as a Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor, by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.,PTM:Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.,simila
  • Species: Human;Rat;Mouse;
  • Range: WB;ELISA
  • Protein: Krueppel-like factor 1
  • DA: 38kD
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