Wide-range ELISA Kit for Salusin Beta (SALb)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

24.69-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 8.87pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Salusin Beta (SALb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Wide-range Salusin Beta (SALb) and unlabeled Wide-range Salusin Beta (SALb) (Standards or samples) with the pre-coated antibody specific to Wide-range Salusin Beta (SALb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Wide-range Salusin Beta (SALb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Wide-range Salusin Beta (SALb) in the sample.

Alternative Names

Item Name

Salusin Beta

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;

Reference