Wide-range ELISA Kit for Glycated Hemoglobin A1c (HbA1c)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1000ug/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than

Sample type

plasma and erythrocyte lysates

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Glycated Hemoglobin A1c (HbA1c) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Wide-range Glycated Hemoglobin A1c (HbA1c) and unlabeled Wide-range Glycated Hemoglobin A1c (HbA1c) (Standards or samples) with the pre-coated antibody specific to Wide-range Glycated Hemoglobin A1c (HbA1c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Wide-range Glycated Hemoglobin A1c (HbA1c) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Wide-range Glycated Hemoglobin A1c (HbA1c) in the sample.

Alternative Names

Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Item Name

Glycated Hemoglobin A1c

Research Area

Reference