Wide-range ELISA Kit for D-Dimer (D2D)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.53ng/mL

Sample type

plasma

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range D-Dimer (D2D) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Wide-range D-Dimer (D2D) and unlabeled Wide-range D-Dimer (D2D) (Standards or samples) with the pre-coated antibody specific to Wide-range D-Dimer (D2D). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Wide-range D-Dimer (D2D) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Wide-range D-Dimer (D2D) in the sample.

Alternative Names

D 2 Dimer

Item Name

D-Dimer

Research Area

Hematology;Cardiovascular biology;

Reference