Wide-range ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.73pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) and unlabeled Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) (Standards or samples) with the pre-coated antibody specific to Wide-range Amyloid Beta Peptide 1-42 (Ab1-42). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Wide-range Amyloid Beta Peptide 1-42 (Ab1-42) in the sample.

Alternative Names

Item Name

Amyloid Beta Peptide 1-42

Research Area

Neuro science;

Reference