VRK2 Antibody

Size

100ug

Clone Number

Aliases

2810003O05Rik antibody; AI447698 antibody; EC 2.7.11.1 antibody; Serine/threonine protein kinase VRK2 antibody; Serine/threonine-protein kinase VRK2 antibody; Vaccinia related kinase 2 antibody; Vaccinia virus B1R related kinase 2 antibody; Vaccinia-related kinase 2 antibody; VRK 2 antibody; VRK2 antibody; VRK2_HUMAN antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q86Y07

Immunogen

Recombinant Human Serine/threonine-protein kinase VRK2 protein (317-472AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC, IF; Recommended dilution: IHC:1:500-1:1000, IF:1:200-1:500

Background

Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates \\\'Thr-18\\\' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

VRK2

Research Areas

Signal transduction