vpx Antibody

Size

100ul

Clone Number

Aliases

vpxProtein Vpx antibody; Viral protein X antibody; X ORF protein antibody

Immunogen Species

Homo sapiens (Human) immunodeficiency virus type 2 subtype A

UniProt ID

P06939

Immunogen

Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpx protein (1-112AA)

Raised in

Rabbit

Species Reactivity

Human immunodeficiency virus type 2 subtype A

Tested Applications

ELISA, WB; Recommended dilution: WB:1:500-1:5000

Background

Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (By similarity).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

vpx

Research Areas

N/A