VPS4A Antibody

Size

100ul

Clone Number

Aliases

hVPS4 antibody; Protein SKD2 antibody; SKD1 antibody; SKD1 homolog antibody; SKD1A antibody; SKD2 antibody; Vacuolar protein sorting 4 homolog A antibody; Vacuolar protein sorting 4, yeast, homolog of, A antibody; Vacuolar protein sorting factor 4A antibody; Vacuolar protein sorting-associated protein 4A antibody; vacuolar sorting protein 4 antibody; VPS4 antibody; VPS4-1 antibody; Vps4a antibody; VPS4A_HUMAN antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9UN37

Immunogen

Recombinant Human Vacuolar protein sorting-associated protein 4A protein (1-120AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

Background

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

VPS4A

Research Areas

Cell biology; Microbiology; Signal transduction