VPS35 Antibody

Size

100ul

Clone Number

Aliases

DKFZp434E1211 antibody; DKFZp434P1672 antibody; FLJ10752 antibody; FLJ13588 antibody; FLJ20388 antibody; hVPS35 antibody; Maternal embryonic 3 antibody; Maternal-embryonic 3 antibody; MEM 3 antibody; MEM3 antibody; PARK17 antibody; TCCCTA00141 antibody; Vacuolar protein sorting 35 (yeast) antibody; Vacuolar protein sorting 35 antibody; Vacuolar protein sorting 35 homolog antibody; Vacuolar protein sorting associated protein 35 antibody; Vacuolar protein sorting-associated protein 35 antibody; Vesicle protein sorting 35 antibody; VPS 35 antibody; VPS35 antibody; VPS35_HUMAN antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q96QK1

Immunogen

Recombinant Human Vacuolar protein sorting-associated protein 35 protein (1-250AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Background

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of FAM21C (PubMed:22070227). Mediates the association of the CSC with the WASH complex via FAM21 (PubMed:22070227, PubMed:24980502, PubMed:24819384). Required for the endosomal localization of TBC1D5 (PubMed:20923837).

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

VPS35

Research Areas

Signal transduction