USP10 Antibody

Size

100ug

Clone Number

Aliases

Deubiquitinating enzyme 10 antibody; KIAA0190 antibody; MGC124997 antibody; MGC2621 antibody; Ubiquitin carboxyl terminal hydrolase 10 antibody; Ubiquitin carboxyl-terminal hydrolase 10 antibody; Ubiquitin specific peptidase 10 antibody; Ubiquitin specific processing protease 10 antibody; Ubiquitin specific protease 10 antibody; Ubiquitin thioesterase 10 antibody; Ubiquitin thiolesterase 10 antibody; Ubiquitin-specific-processing protease 10 antibody; UBP10_HUMAN antibody; UBPO antibody; USP 10 antibody; USP10 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q14694

Immunogen

Recombinant Human Ubiquitin carboxyl-terminal hydrolase 10 protein (453-592AA)

Raised in

Rabbit

Species Reactivity

Human, Rat

Tested Applications

ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Background

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

USP10

Research Areas

Epigenetics and Nuclear Signaling; Cell biology