TXK Antibody

Size

100ug

Clone Number

Aliases

BTKL antibody; EC 2.7.10.2 antibody; MGC22473 antibody; Protein-tyrosine kinase 4 antibody; PSCTK5 antibody; PTK4 antibody; Resting lymphocyte kinase antibody; RLK antibody; TKL antibody; TXK antibody; TXK tyrosine kinase antibody; TXK_HUMAN antibody; Tyrosine protein kinase TXK antibody; Tyrosine-protein kinase TXK antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

P42681

Immunogen

Recombinant Human Tyrosine-protein kinase TXK protein (32-83AA)

Raised in

Rabbit

Species Reactivity

Human, Rat

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Background

Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates \\\'Tyr-201\\\' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

TXK

Research Areas

Epigenetics and Nuclear Signaling; Immunology; Signal transduction