TUBB Monoclonal Antibody

Size

Clone Number

Aliases

beta 5-tubulin; beta Ib tubulin; beta-4 tubulin; M40; MGC117247; MGC16435; OK/SW-cl.56; TBB5; TUBB; TUBB1; TUBB5

Immunogen Species

Zebrafish

UniProt ID

P07437

Immunogen

Recombinant Protein

Raised in

Mouse

Species Reactivity

Zebrafish

Tested Applications

ELISA,WB,IHC,IF;Recommended dilution:WB:1:500-1:5000,IHC:1:50-1:500,IF:1:50-1:200

Background

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of Beta-Tubulin may not be stable in certain cells. For example, expression of Beta-Tubulin in adipose tissue is very low and therefore Beta-Tubulin should not be used as loading control for these tissues.

Clonality

Monoclonal

Isotype

IgG1

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

TUBB

Research Areas

Isotype/Loading Controls;Tags & Cell Markers;Signal transduction