TRIM21 Antibody

Size

100ug

Clone Number

Aliases

52 kDa ribonucleoprotein autoantigen Ro/SS-A antibody; 52 kDa Ro protein antibody; 52kD Ro/SSA autoantigen antibody; Autoantigen Ro/SSA, 52-KD antibody; E3 ubiquitin-protein ligase TRIM21 antibody; RING finger protein 81 antibody; RNF81 antibody; Ro 52 antibody; Ro(SS-A) antibody; Ro52 antibody; RO52_HUMAN antibody; Sicca syndrome antigen A antibody; Sjoegren syndrome type A antigen antibody; Sjogren syndrome antigen A1 antibody; Sjogren syndrome type A antigen antibody; SS-A antibody; SSA antibody; SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro) antibody; TRIM21 antibody; Tripartite motif protein TRIM21 antibody; Tripartite motif-containing 21 antibody; Tripartite motif-containing protein 21 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

P19474

Immunogen

Recombinant Human E3 ubiquitin-protein ligase TRIM21 protein (1-475AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200

Background

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B (\\\'Thr-187\\\' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses (PubMed:26347139).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

TRIM21

Research Areas

Epigenetics and Nuclear Signaling; Immunology