TREX1 Antibody

Size

100ul

Clone Number

Aliases

TREX1 antibody; Three-prime repair exonuclease 1 antibody; EC 3.1.11.2 antibody; 3'-5' exonuclease TREX1 antibody; Deoxyribonuclease III antibody; DNase III antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9NSU2

Immunogen

Recombinant Human Three-prime repair exonuclease 1 protein (1-270AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Background

Major cellular 3\\\'-to-5\\\' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3\\\' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3\\\' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

TREX1

Research Areas

Epigenetics and Nuclear Signaling; Immunology