TOP1MT Antibody, HRP conjugated

Size

100ug

Clone Number

Aliases

TOP1MT antibody; DNA topoisomerase I antibody; mitochondrial antibody; TOP1mt antibody; EC 5.6.2.1 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q969P6

Immunogen

Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA

Background

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3\'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5\'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5\'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

HRP

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

TOP1MT

Research Areas

Epigenetics and Nuclear Signaling