TOP1MT Antibody

Size

100ug

Clone Number

Aliases

TOP1MT antibody; DNA topoisomerase I antibody; mitochondrial antibody; TOP1mt antibody; EC 5.6.2.1 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q969P6

Immunogen

Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:500-1:1000

Background

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3\\\'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5\\\'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5\\\'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

TOP1MT

Research Areas

Epigenetics and Nuclear Signaling