THOC1 Antibody

Size

100ul

Clone Number

Aliases

hTREX84 antibody; Death domain containing protein p84N5 antibody; HPR 1 antibody; HPR1 antibody; hTREX84 antibody; Nuclear matrix protein p84 antibody; P84 antibody; p84N5 antibody; Tho 1 antibody; THO complex 1 antibody; THO complex subunit 1 antibody; Tho1 antibody; THOC 1 antibody; Thoc1 antibody; THOC1_HUMAN antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q96FV9

Immunogen

Recombinant Human THO complex subunit 1 protein (428-657AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB, IHC, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

Background

Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5\\\' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi\\\'s sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

THOC1

Research Areas

Epigenetics and Nuclear Signaling; Tags & Cell Markers