TERF2 Antibody

Size

100ug

Clone Number

Aliases

Telomeric DNA binding protein antibody; Telomeric DNA-binding protein antibody; Telomeric repeat binding factor 2 antibody; Telomeric repeat binding protein 2 antibody; Telomeric repeat-binding factor 2 antibody; TERF 2 antibody; Terf2 antibody; TERF2_HUMAN antibody; TRBF 2 antibody; TRBF2 antibody; TRF 2 antibody; TRF2 antibody; TTAGGG repeat binding factor 2 antibody; TTAGGG repeat-binding factor 2 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q15554

Immunogen

Recombinant Human Telomeric repeat-binding factor 2 protein (78-238AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Background

Binds the telomeric double-stranded 5\\\'-TTAGGG-3\\\' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5\\\'-TTAGGG-3\\\' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3\\\' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

TERF2

Research Areas

Epigenetics and Nuclear Signaling; Cancer