TDG Antibody

Size

100ul

Clone Number

Aliases

C JUN leucine zipper interactive protein antibody; C JUN leucine zipper interactive protein antibody; C-JUN leucine zipper interactive protein JZA-3 antibody; E130317C12Rik antibody; EC 3.2.2.29 antibody; G/T mismatch specific thymine DNA glycosylase antibody; G/T mismatch specific thymine DNA glycosylase antibody; G/T mismatch-specific thymine DNA glycosylase antibody; JZA 3 antibody; Jza1 antibody; T:G mismatch thymine glycosylase antibody; Tdg antibody; TDG_HUMAN antibody; Thymine DNA glycosylase antibody; Thymine-DNA glycosylase antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q13569

Immunogen

Recombinant Human G/T mismatch-specific thymine DNA glycosylase protein (141-410AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200

Background

DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

TDG

Research Areas

Epigenetics and Nuclear Signaling