STT3B Antibody

Size

100ug

Clone Number

Aliases

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B antibody; Homolog of yeast STT3 antibody; Oligosaccharyl transferase subunit STT3B antibody; SIMP antibody; source of immunodominant MHC associated peptides antibody; Source of immunodominant MHC-associated peptides homolog antibody; STT3 subunit of the oligosaccharyltransferase complex homolog B (S. cerevisiae) antibody; STT3 subunit of the oligosaccharyltransferase complex homolog B antibody; STT3-B antibody; Stt3b antibody; STT3B_HUMAN antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q8TCJ2

Immunogen

Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B protein (682-809AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:200-1:500

Background

Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient post-translational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation. Mediates glycosylation of the disease variant AMYL-TTR \\\'Asp-38\\\' of TTR at \\\'Asn-118\\\', leading to its degradation.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

STT3B

Research Areas

Metabolism; Signal transduction