STK3 Antibody

Size

100ul

Clone Number

Aliases

0610042I06Rik antibody; EC 2.7.11.1 antibody; FLJ90748 antibody; KB 1458E12.1 antibody; Kinase responsive to stress 1 antibody; KRS1 antibody; Mammalian STE20 like protein kinase 2 antibody; Mammalian STE20-like protein kinase 2 antibody; Mammalian sterile 20-like 2 antibody; Mess1 antibody; MST antibody; MST-2 antibody; MST2 antibody; Mst3 antibody; Serine/threonine kinase 3 (STE20 homolog, yeast) antibody; Serine/threonine kinase 3 (Ste20, yeast homolog) antibody; Serine/threonine kinase 3 antibody; Serine/threonine protein kinase 3 antibody; Serine/threonine protein kinase Krs1 antibody; Serine/threonine-protein kinase 3 antibody; Serine/threonine-protein kinase Krs-1 antibody; STE20 like kinase MST2 antibody; STE20-like kinase MST2 antibody; Stk3 antibody; STK3_HUMAN antibody; wu:fc19e11 antibody; zgc:55383 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q13188

Immunogen

Recombinant Human Serine/threonine-protein kinase 3 protein (323-450AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Background

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on \\\'Ser-259\\\'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on \\\'Thr-74\\\'. Acts cooperatively with MOBKL1B to activate STK38.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

STK3

Research Areas

Cell biology; Signal transduction