SPPL3 Antibody

Size

100ug

Clone Number

Aliases

SPPL3 antibody; IMP2 antibody; PSL4 antibody; Signal peptide peptidase-like 3 antibody; SPP-like 3 antibody; EC 3.4.23.- antibody; Intramembrane protease 2 antibody; IMP-2 antibody; Presenilin homologous protein 1 antibody; PSH1 antibody; Presenilin-like protein 4 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q8TCT6

Immunogen

Recombinant Human Signal peptide peptidase-like 3 protein (213-263AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IF; Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200

Background

Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries (PubMed:16873890, PubMed:25354954, PubMed:25827571). Acts like a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1 (PubMed:25354954, PubMed:25827571). Catalyzes the intramembrane cleavage of the envelope glycoprotein gp130 and/or the leader peptide gp18LP of the simian foamy virus independent of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis (PubMed:23132852). May also have the ability to serve as a shedding protease for subsequent intramembrane proteolysis by SPPL2A and SPPL2B of the envelope glycoprotein gp130 (PubMed:23132852). Plays a role in the regulation of cellular glycosylation processes (PubMed:25354954). Required to link T-cell antigen receptor (TCR) and calcineurin-NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease-independent manner (PubMed:25384971).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

SPPL3

Research Areas

Cell biology