SKA2 Antibody

Size

100ug

Clone Number

Aliases

FAM33A antibody; Family with sequence similarity 33; member A antibody; FLJ12758 antibody; MGC110975 antibody; Protein FAM33A antibody; SKA 2 antibody; SKA2 antibody; SKA2_HUMAN antibody; Spindle and kinetochore associated complex subunit 2 antibody; Spindle and kinetochore associated protein 2 antibody; Spindle and kinetochore-associated protein 2 antibody; Spindle and KT (kinetochore) associated 2 antibody; Spindle and KT associated 2 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q8WVK7

Immunogen

Recombinant Human Spindle and kinetochore-associated protein 2 protein (1-121AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:200-1:500

Background

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:17093495, PubMed:19289083). In the complex, it is required for SKA1 localization (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

SKA2

Research Areas

Cell biology