SH2B1 Antibody

Size

100ul

Clone Number

Aliases

DKFZp547G1110 antibody; FLJ30542 antibody; KIAA1299 antibody; PH and SH2 domain-containing signaling mediator antibody; Pro-rich antibody; Pro-rich, PH and SH2 domain-containing signaling mediator antibody; PSM antibody; SH2 B antibody; SH2 domain-containing protein 1B antibody; SH2 domain-containing putative adapter SH2-B antibody; SH2-B signaling protein antibody; SH2B antibody; SH2B adapter protein 1 antibody; SH2B adaptor protein 1 antibody; Sh2b1 antibody; SH2B1 protein antibody; SH2B1_HUMAN antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9NRF2

Immunogen

Recombinant Human SH2B adapter protein 1 protein (360-520AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Background

Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin (INS), insulin-like growth factor I (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF) and fibroblast growth factors (FGFs). In growth hormone (GH) signaling, autophosphorylated (\\\'Tyr-813\\\') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin (LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway; prolongs NGF-induced phosphorylation of AKT1 on \\\'Ser-473\\\' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity. Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis (By similarity).

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

SH2B1

Research Areas

Epigenetics and Nuclear Signaling; Metabolism; Signal transduction