SESN2 Antibody

Size

100ul

Clone Number

Aliases

DKFZp761M0212 antibody; DKFZp761M02121 antibody; Hi95 antibody; Hypoxia induced gene 95 antibody; MGC11758 antibody; OTTHUMP00000003641 antibody; OTTMUSP00000010184 antibody; RP23 63D8.3 antibody; SES2 antibody; SESN 2 antibody; SESN2 antibody; SESN2_HUMAN antibody; SEST2 antibody; Sestrin 2 antibody; Sestrin-2 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

P58004

Immunogen

Peptide sequence from Human Sestrin-2 protein (42-61AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IF; Recommended dilution: IF:1:50-1:200

Background

Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling (PubMed:18692468, PubMed:25263562, PubMed:25457612, PubMed:26449471, PubMed:26612684, PubMed:26586190). Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:26449471, PubMed:26586190). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1 (PubMed:24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed:18692468). Has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (PubMed:26612684). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (PubMed:19113821).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

SESN2

Research Areas

Cardiovascular; Cell biology; Metabolism