SATB1 Antibody

Size

100ul

Clone Number

Aliases

DNA binding protein SATB1 antibody; DNA-binding protein SATB1 antibody; SATB homeobox 1 antibody; SATB1 antibody; SATB1_HUMAN antibody; Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody; Special AT rich sequence binding protein 1 antibody; Special AT-rich sequence-binding protein 1 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q01826

Immunogen

Recombinant Human DNA-binding protein SATB1 protein (484-763AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB; Recommended dilution: WB:1:500-1:2000

Background

Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

SATB1

Research Areas

Epigenetics and Nuclear Signaling