RTF1 Antibody

Size

100ug

Clone Number

Aliases

CDG1N antibody; GTL 7 antibody; GTL7 antibody; KIAA0252 antibody; Ortholog of mouse gene trap locus 7 antibody; RNA polymerase associated protein RTF1 homolog antibody; RNA polymerase-associated protein RTF1 homolog antibody; RTF 1 antibody; RTF1 antibody; Rtf1 Paf1/RNA polymerase II complex component homolog antibody; RTF1_HUMAN antibody; rtf1L antibody; YGL244W antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q92541

Immunogen

Recombinant Human RNA polymerase-associated protein RTF1 homolog protein (620-670AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Background

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and \'Ser-2\'- and \'Ser-5\'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 \'Lys-4\' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of \'Lys-120\' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3\' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 \'Lys-4\' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex (By similarity).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

RTF1

Research Areas

Epigenetics and Nuclear Signaling; Microbiology; Stem cells