RPTOR Antibody

Size

100ug

Clone Number

Aliases

KIAA1303 antibody; KOG1 antibody; Mip1 antibody; P150 target of rapamycin (TOR) scaffold protein antibody; p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody; P150 target of rapamycin (TOR)-scaffold protein antibody; Raptor antibody; Regulatory associated protein of mTOR antibody; Regulatory associated protein of MTOR complex 1 antibody; Regulatory-associated protein of mTOR antibody; RPTOR antibody; RPTOR_HUMAN antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q8N122

Immunogen

Recombinant Human Regulatory-associated protein of mTOR protein (13-128AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

Background

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at \\\'Thr-389\\\', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

RPTOR

Research Areas

Epigenetics and Nuclear Signaling; Cell biology; Signal transduction