RPA1 Monoclonal Antibody

Size

Clone Number

Aliases

Dmrpa1; Drosophila Replication Protein A; DRPA; HSSB; Human single stranded DNA binding protein; MST075; MSTP075; p70; REPA1; Replication factor A; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit;

Immunogen Species

Human

UniProt ID

P27694

Immunogen

Purified recombinant human RPA70 protein fragments expressed in E.coli.

Raised in

Mouse

Species Reactivity

Human,Monkey,Mouse,Rat

Tested Applications

ELISA,WB,ICC,IP;Recommended dilution:WB:1:500-1:5000,ICC:1:50-1:200IP:1:200-1:2000

Background

Plays an essential role in several cellular processes in DNA metabolism including replication,recombination and DNA repair.Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA).aRPA binds single-stranded DNA and probably plays a role in DNA repair;it does not support chromosomal DNA replication and cell cycle progression through S-phase.In vitro,aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC),the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Clonality

Monoclonal

Isotype

IgG2a

Purification Method

Affinity purified

Conjugate

Non-conjugated

Buffer

Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Form

liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

RPA1

Research Areas

Epigenetics and Nuclear Signaling