RBX1 Antibody

Size

100ul

Clone Number

Aliases

BA554C12.1 antibody; E3 ubiquitin-protein ligase RBX1 antibody; FLJ60363 antibody; MGC13357 antibody; MGC1481 antibody; OTTHUMP00000028983 antibody; Protein ZYP antibody; Rbx 1 antibody; Rbx1 antibody; RBX1_HUMAN antibody; Regulator of cullins 1 antibody; Ring box 1 antibody; Ring box 1 E3 ubiquitin protein ligase antibody; RING box protein 1 antibody; RING finger protein 75 antibody; RING finger protein antibody; RING-box protein 1 antibody; Ringbox protein 1 antibody; RNF 75 antibody; RNF75 antibody; ROC 1 antibody; ROC1 antibody; ZYP protein antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

P62877

Immunogen

Recombinant Human E3 ubiquitin-protein ligase RBX1 protein (1-108AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200

Background

E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

RBX1

Research Areas

Epigenetics and Nuclear Signaling; Cell biology