RAG-2 Monoclonal Antibody

Ab type

Primary antibody

Background

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodef

Reactivity

Human

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Immunogen

Purified recombinant fragment of human RAG-2 (350-527aa) expressed in E. Coli.

Storage Stability

-20°C for one year

Concentration

1 mg/ml

MolecularWeight_Da

N/A

synonyms

RAG2; V(D)J recombination-activating protein 2; RAG-2