RAD1 Antibody

Size

100ul

Clone Number

Aliases

Cell cycle checkpoint protein Hrad1 antibody; Cell cycle checkpoint protein Rad 1 A / B antibody; Cell cycle checkpoint protein RAD1 antibody; Checkpoint control protein HRAD1 antibody; Checkpoint control protein RAD1 antibody; DNA repair exonuclease antibody; DNA repair exonuclease rad1 antibody; DNA repair exonuclease rad1 homolog antibody; DNA repair exonuclease REC1 antibody; DNA repair protein RAD1 antibody; EC 3.1.11.2 antibody; Exonuclease homolog RAD1 antibody; GTP-binding protein RAD antibody; hRAD 1 antibody; hRAD1 antibody; MGC77779 antibody; RAD 1 antibody; RAD antibody; RAD1 antibody; RAD1 homolog (S. pombe) antibody; RAD1 homolog antibody; Rad1 like DNA damage checkpoint antibody; Rad1 like DNA damage checkpoint protein antibody; RAD1; S. pombe; homolog of antibody; Rad1-like DNA damage checkpoint protein antibody; RAD1_HUMAN antibody; Ras associated with diabetes antibody; REC 1 antibody; REC1 antibody; RRAD antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

O60671

Immunogen

Recombinant Human Cell cycle checkpoint protein RAD1 protein (1-282AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200

Background

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3\\\'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3\\\'->5\\\' double stranded DNA exonuclease activity.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

RAD1

Research Areas

Epigenetics and Nuclear Signaling