PUF60 Antibody

Size

100ug

Clone Number

Aliases

60 kDa poly(U)-binding-splicing factor antibody; FBP-interacting repressor antibody; FIR antibody; FLJ31379 antibody; FUSE-binding protein-interacting repressor antibody; Poly U binding splicing factor 60KDa antibody; Poly(U) binding splicing factor 60 antibody; Poly(U)-binding-splicing factor PUF60 antibody; poly-U binding splicing factor PUF60 antibody; PUF60 antibody; PUF60_HUMAN antibody; pyrimidine tract binding splicing factor antibody; Ro ribonucleoprotein-binding protein 1 antibody; Ro-binding protein 1 antibody; RoBP1 antibody; RoBPI antibody; siah binding protein 1 antibody; Siah-binding protein 1 antibody; Siah-BP1 antibody; SIAHBP1 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9UHX1

Immunogen

Recombinant Human Poly(U)-binding-splicing factor PUF60 protein (302-413AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200

Background

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3\\\'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3\\\'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

PUF60

Research Areas

Epigenetics and Nuclear Signaling