POLR3E Antibody

Size

100ug

Clone Number

Aliases

POLR3E antibody; KIAA1452 antibody; DNA-directed RNA polymerase III subunit RPC5 antibody; RNA polymerase III subunit C5 antibody; DNA-directed RNA polymerase III 80 kDa polypeptide antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9NVU0

Immunogen

Recombinant Human DNA-directed RNA polymerase III subunit RPC5 protein (1-260AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

POLR3E

Research Areas

Epigenetics and Nuclear Signaling