POLI Antibody

Size

100ug

Clone Number

Aliases

DNA polymerase iota antibody; Eta 2 antibody; Eta2 antibody; POLI antibody; POLI_HUMAN antibody; Polymerase (DNA directed) iota antibody; RAD 30B antibody; RAD30 homolog B antibody; RAD30, S. cerevisiae, homolog of antibody; RAD30B antibody; RAD3OB antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9UNA4

Immunogen

Recombinant Human DNA polymerase iota protein (458-711AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

Background

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5\'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

POLI

Research Areas

Epigenetics and Nuclear Signaling