POLH Antibody

Size

100ug

Clone Number

Aliases

DNA polymerase eta antibody; FLJ16395 antibody; FLJ21978 antibody; Polh antibody; POLH_HUMAN antibody; polymerase DNA directed eta antibody; RAD30 antibody; RAD30 homolog A antibody; RAD30A antibody; Xeroderma pigmentosum variant type protein antibody; XP V antibody; XPV antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9Y253

Immunogen

Recombinant Human DNA polymerase eta protein (408-609AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Background

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5\\\'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

POLH

Research Areas

Epigenetics and Nuclear Signaling