PODXL Monoclonal Antibody

Size

100ul

Clone Number

4D2G7

Aliases

Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1

Immunogen Species

Human

UniProt ID

O00592

Immunogen

Recombinant Human PODXL protein

Raised in

Mouse

Species Reactivity

Human, Mouse, Rat

Tested Applications

ELISA, WB, IHC, IP, IF, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200, IP:1:200-1:2000

Background

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

Clonality

Monoclonal

Isotype

IgG2b

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

PODXL

Research Areas

Cancer; Cardiovascular; Signal transduction; Stem cells