PKM Monoclonal Antibody

Size

100ul

Clone Number

4C8E12

Aliases

CTHBP antibody; Cytosolic thyroid hormone-binding protein antibody; KPYM_HUMAN antibody; OIP-3 antibody; Opa-interacting protein 3 antibody; p58 antibody; pkm antibody; PKM1 antibody; PKM2 antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase muscle isozyme antibody; Pyruvate kinase PKM antibody; THBP1 antibody; Thyroid hormone-binding protein 1 antibody; Tumor M2-PK antibody

Immunogen Species

Human

UniProt ID

P14618

Immunogen

Recombinant Human Pyruvate kinase PKM protein (2-531AA)

Raised in

Mouse

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB, IHC, IF, FC, IP; Recommended dilution: WB: 1:4000-1:512000, IHC: 1:500-1:1000, IF: 1:150-1:300, FC: 1:50-1:100, IP: 1µl-4µl

Background

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Clonality

Monoclonal

Isotype

IgG1

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

PKM

Research Areas

Immunology