PIM1 Antibody

Size

100ug

Clone Number

Aliases

Oncogene PIM 1 antibody; Oncogene PIM1 antibody; PIM 1 antibody; pim 1 kinase 44 kDa isoform antibody; Pim 1 kinase antibody; pim 1 oncogene (proviral integration site 1) antibody; Pim 1 oncogene antibody; PIM antibody; PIM1 antibody; pim1 kinase 44 kDa isoform antibody; PIM1_HUMAN antibody; Pim2 antibody; PIM3 antibody; Proto oncogene serine/threonine protein kinase Pim 1 antibody; Proto-oncogene serine/threonine-protein kinase Pim-1 antibody; Proviral integration site 1 antibody; Proviral integration site 2 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

P11309

Immunogen

Recombinant Human Serine/threonine-protein kinase pim-1 protein (25-86AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

Background

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

PIM1

Research Areas

Epigenetics and Nuclear Signaling; Cancer; Signal transduction; Stem cells