Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Antibody

Size

100ul

Clone Number

2D1

Aliases

Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4, PAK4, KIAA1142

Immunogen Species

Human

UniProt ID

O96013/Q9P286/Q9NQU5

Immunogen

A synthesized peptide derived from human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602)

Raised in

Species Reactivity

Human

Tested Applications

ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200

Background

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

Clonality

Monoclonal

Isotype

Rabbit IgG

Purification Method

Affinity-chromatography

Conjugate

Non-conjugated

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

PAK4/PAK5/PAK6

Research Areas

Cell Biology