Phospho-Histone H2AX (S139) Antibody

Size

100ul

Clone Number

1F10

Aliases

Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX

Immunogen Species

Human

UniProt ID

P16104

Immunogen

A synthesized peptide

Raised in

Species Reactivity

Human

Tested Applications

ELISA, WB; Recommended dilution: WB:1:3000-1:10000

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Clonality

Monoclonal

Isotype

Rabbit IgG

Purification Method

Affinity-chromatography

Conjugate

Non-conjugated

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

H2AFX

Research Areas

Epigenetics and Nuclear Signaling