pfo Antibody

Size

100ug

Clone Number

Aliases

pfo antibody; pfoA antibody; pfoR antibody; CPE0163 antibody; Perfringolysin O antibody; Theta-toxin antibody; Thiol-activated cytolysin antibody

Immunogen Species

Clostridium perfringens

UniProt ID

P0C2E9

Immunogen

Recombinant Clostridium perfringens Perfringolysin O protein (29-500AA)

Raised in

Rabbit

Species Reactivity

Clostridium perfringens

Tested Applications

ELISA, WB; Recommended dilution: WB:1:500-1:5000

Background

Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

pfo

Research Areas

Others