PAK1 Antibody

Size

100ul

Clone Number

Aliases

ADRB2 antibody; Alpha PAK antibody; Alpha-PAK antibody; MGC130000 antibody; MGC130001 antibody; p21 activated kinase 1 antibody; p21 protein (Cdc42/Rac) activated kinase 1 antibody; p21-activated kinase 1 antibody; p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody; p65 PAK antibody; p65-PAK antibody; P68-PAK antibody; PAK alpha antibody; PAK-1 antibody; Pak1 antibody; PAK1_HUMAN antibody; Paka antibody; PAKalpha antibody; Protein kinase MUK2 antibody; Rac/p21-activated kinase antibody; Serine/threonine-protein kinase PAK 1 antibody; STE20 homolog yeast antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q13153

Immunogen

Recombinant Human Serine/threonine-protein kinase PAK 1 protein (1-240AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Background

Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at \\\'Ser-338\\\' and \\\'Ser-339\\\' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at \\\'Ser-246\\\' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

PAK1

Research Areas

Cancer; Cell biology; Signal transduction