NSJ Bioreagents
SKU:V9737-100UG
Serum Response Factor Antibody / SRF, 100 ug
Serum Response Factor Antibody / SRF, 100 ug
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Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.
Specifications
| Catalog No | V9737-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | PCRP-SRF-1F7 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b |
| Species Reactivity | Human |
| Application | ELISA, IF, WB, FACS, IHC-P |
| Application Details | ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml |
| Application Note | Optimal dilution of the Serum Response Factor antibody should be determined by the researcher. |
| Localization | Nucleus |
| Immunogen | Recombinant full-length human protein was used as the immunogen for the Serum Response Factor antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the Serum Response Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Serum Response Factor antibody is available for research use only. |
| Uniprot # | P11831 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/serum-response-factor-antibody-srf-pcrp-srf-1f7-v9737 |
| Title | Serum Response Factor Antibody / SRF |
| Description | Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases. |
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