NSJ Bioreagents
SKU:V9723-100UG
ERK2 Antibody / MAPK1, 100 ug
ERK2 Antibody / MAPK1, 100 ug
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Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely-related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development.
Specifications
| Catalog No | V9723-100UG |
|---|---|
| Family | Primary antibody |
| Qty | 100 ug |
| Formulation | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Clone | PCRP-MAPK1-1D1 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b |
| Species Reactivity | Human |
| Application | ELISA, FACS, IF |
| Application Details | ELISA (order BSA-free format for coating),Flow cytometry: 0.5-2ug/million cells,Immunofluorescence: 1-2ug/ml |
| Application Note | Optimal dilution of the ERK2 antibody should be determined by the researcher. |
| Localization | Cytoplasm, Nucleus |
| Immunogen | Recombinant full-length human protein was used as the immunogen for the ERK2 antibody. |
| Purity | Protein A/G affinity |
| Storage | Aliquot the ERK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This ERK2 antibody is available for research use only. |
| Uniprot # | P28482 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/erk2-antibody-mapk1-pcrp-mapk1-1d1-v9723 |
| Title | ERK2 Antibody / MAPK1 |
| Description | Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely-related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. |
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