• IHC staining of FFPE human thyroid tissue with Beta Actin (clone ACTB/1108). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9713-100UG

Beta Actin Antibody / ACTB, 100 ug

Beta Actin Antibody / ACTB, 100 ug

$559.00 USD
$559.00 USD
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All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas -Actin and g-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Specifications

Catalog No V9713-100UG
Family Primary antibody
Qty 100 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone ACTB/1108
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P
Application Details Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the Beta Actin antibody should be determined by the researcher.
Localization Cytoplasm
Immunogen Recombinant human ACTB protein was used as the immunogen for the Beta Actin antibody.
Purity Protein A/G affinity
Storage Aliquot the Beta Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Beta Actin antibody is available for research use only.
Uniprot # P60709
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/beta-actin-antibody-actb-actb1108-v9713
Title Beta Actin Antibody / ACTB
Description All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas -Actin and g-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
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