• IHC staining of FFPE human melanoma tissue with NGFR/TNFRSF16 antibody (clone NGFR/4919) at 2ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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NSJ Bioreagents

V9706-20UG

NGFR/TNFRSF16 Antibody / p75NTR / CD271, 20 ug

NGFR/TNFRSF16 Antibody / p75NTR / CD271, 20 ug

$259.00 USD
$259.00 USD
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It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Specifications

Catalog No V9706-20UG
Family Primary antibody
Qty 20 ug
Formulation 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format Purified
Clone NGFR/4919
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Species Reactivity Human
Application IHC-P
Application Details Immunohistochemistry (FFPE): 1-2ug/ml
Application Note Optimal dilution of the NGFR/TNFRSF16 antibody should be determined by the researcher.
Localization Cell surface, Cytoplasm
Immunogen WM245 melanoma cells of human origin were used as the immunogen for the NGFR/TNFRSF16 antibody.
Purity Protein A/G affinity
Storage Aliquot the NGFR/TNFRSF16 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This NGFR/TNFRSF16 antibody is available for research use only.
Uniprot # P08138
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/ngfrtnfrsf16-antibody-p75ntr-cd271-ngfr4919-v9706
Title NGFR/TNFRSF16 Antibody / p75NTR / CD271
Description It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.
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